NCI-H358 is a tumorigenic cell line that was established in 1981 from the bronchiole tissue of a male with bronchioalveolar carcinoma. NCI-H358 cells display adherent cultural properties in vitro and an epithelial morphology. NCI-H358 produces lung surfactant associated protein A (SP-A) and expresses the RNA of SP-A. NCI-H358 cells are suitable transfection hosts, and Altogen Biosystems offers a NCI-H358 transfection reagent to transfect NCI-H358 cells. The NCI-H358 cell line can be used to study lung cancer and develop therapeutics.